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Microdissection techniques and direct fluorescent antibody staining were used to demonstrate scrub typhus nckettslae in all stages of infected Leptotrombidium (Leptotrombidium) fletcheri (=Malaysian L. akamushi). Rickettsiae were detected in some gut and hemolymph samples from each stage of mite development. Unfed larvae had the highest and protonymphs the lowest numbers of samples positive for rickettsiae. The high incidence of rickettsiae in the salivary glands of unengorgcd larvae along with the low incidence in engorged larvae suggest that rickettsiae in the salivary fluid are the source of infection for the mammalian host. Fluorescent antibody exanunation of eggs and unfed larvae appears to be a practical method of screening field-collected mites for scrub typhus rickettsiae.

Document Type: Research Article

Publication date: September 25, 1975

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  • Journal of Medical Entomology is published bimonthly in January, March, May, July, September, and November. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.

    The journal is divided into the following sections: Morphology, Systematics, Evolution; Sampling, Distribution, Dispersal; Development, Life History; Population and Community Ecology; Behavior, Chemical Ecology; Population Biology/Genetics; Molecular Biology/Genomics; Neurobiology, Physiology, Biochemistry; Vector Control, Pest Management, Resistance, Repellents; Arthropod/Host Interaction, Immunity; Vector/Pathogen/Host Interaction, Transmission; Vector-Borne Diseases, Surveillance, Prevention; Direct Injury, Myiasis, Forensics; Modeling/GIS, Risk Assessment, Economic Impact. In addition to full-length research articles, the journal publishes interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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