Use of Mitochondrial Cytochrome Oxidase I Polymerase Chain Reaction-Restriction Fragment Length Polymorphism for Identifying Subclades of Bemisia tabaci Mediterranean Group
Authors: Chu, Dong; Hu, Xiangshun; Gao, Changsheng; Zhao, Huiyan; Nichols, Robert L.; Li, Xianchun
Source: Journal of Economic Entomology, Volume 105, Number 1, Pages 1-296 , pp. 242-251(10)
Publisher: Entomological Society of America
Abstract:The Mediterranean group (commonly known as Q biotype; hereafter MED) of the sweetpotato whitefly, Bemisia tabaci (Gennadius), originated in the Mediterranean region, but it now has been found in at least 10 countries outside the Mediterranean. Collections of B. tabaci from some of these countries exhibit different pest behaviors and pesticide resistance characteristics, yet all may be classified as MED. A phylogenetic analysis of 120 mitochondrial cytochrome oxidase I (mtCOI) sequences (JN966761‐JN966880) of MED whiteflies collected in Arizona and of 417 retrieved from the GenBank database resolves the MED into five subclades, designated as Q1‐Q5. Only subclades Q1 and Q2 have been detected in the United States. Q1 and the other four subclades (Q2‐Q5) differ in the number or position of the AluI recognition sites. Based on the differences in the AluI recognition sites reported here and the previously reported differences in VspI recognition sites, we developed a simple diagnostic technique to identify subclades Q1‐Q5 by using mtCOI polymerase chain reaction (PCR)-restriction fragment-length polymorphism (RFLP). A test of a worldwide collection of whiteflies demonstrates that this combination mtCOI PCR-RFLP technique can reliably distinguish not only the MED from the Middle East-Asia Minor 1 group but also the Q1 from any of the other four MED subclades.
Document Type: Research Article
Publication date: 2012-02-01
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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