A Medium for the Production of Biopesticides (Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis) in Mosquito Control
Chicken feathers, discarded as bioorganic wastes from poultry processing industries, are rich in keratin (structural protein). Deoiled rice bran (DRB), in contrast, is an agrowaste product consisting of protein, fiber, and carbohydrates. This study highlights a new medium for producing biopesticides from these bioorganic wastes, by culturing Bacillus sphaericus (Bs) and B. thuringiensis subsp. israelensis (Bti), to synthesize mosquitocidal toxins. Biochemical studies indicated that the amount of mosquitocidal spore/crystal toxins produced from the experimental culture medium (chicken feather waste [CFW] + DRB) is higher than that from the conventional medium (Nutrient Yeast Extract Salt Medium [NYSM]). The bacteria produced in these media (NYSM, CFW, DRB, and CFW + DRB) were bioassayed against mosquito vectors, and the toxic effect was found to be statistically similar (fiducial limits overlapping). Cost analysis based on the raw materials, indicated that, the use of CFW and DRB, as culture medium, is highly economical, for the industrial production of these mosquito pathogenic bacilli.
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Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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