Cross-Species Amplification and Polymorphism of Microsatellite Loci in the Soybean Aphid, Aphis glycines

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We tested the utility of 18 previously characterized Aphis spp. microsatellite loci for polymorphism and differentiation among populations of the soybean aphid, Aphis glycines. Loci were chosen from a closely related species (Aphis gossypii) and a more distantly related species (Aphis fabae). We found nine loci to be polymorphic among Korean and North American populations. Overall expected heterozygosity was moderate (average: 0.47; range: 0–1), although populations substantially differed in deviations from Hardy-Weinberg equilibrium. These loci will be valuable in characterizing population differentiation, migration and adaptation in an economically important pest of soybeans.

Keywords: Aphis glycines; cross-species amplification; microsatellites; soybean aphid

Document Type: Short Communication

Publication date: June 1, 2009

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