Fate of a Genetically Modified Bacterium in Foregut of Glassy-Winged Sharpshooter (Hemiptera: Cicadellidae)

Authors: Ramirez, José L.; Perring, Thomas M.; Miller, Thomas A.

Source: Journal of Economic Entomology, Volume 101, Number 5, October 2008 , pp. 1519-1525(7)

Publisher: Entomological Society of America

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Symbiotic control is a new strategy being investigated to prevent the spread of insect-transmitted pathogens by reducing vector competence. We are developing this strategy to reduce the spread of Xylella fastidiosa by Homalodisca vitripennis (Germar) [formerly Homalodisca coagulata (Say)] (Hemiptera: Cicadellidae), the glassy-winged sharpshooter. In this study, the fate of a transformed symbiotic bacterium, Alcaligenes xylosoxidans variety denitrificans (S1Axd), in the foregut of glassy-winged sharpshooter when fed on citrus (Citrus spp.) and grape (Vitris spp.) was assessed. TaqMan-based quantitative real-time polymerase chain reaction (PCR) was used to detect and quantify bacterial cells remaining in the foregut at 0, 2, 4, 9, and 12 d after acquisition. S1Axd titer dropped rapidly by 2 d after acquisition, but in spite of this, at end of the 12-d experimental period, 45 and 38% of the glassy-winged sharpshooters retained the transformed bacteria, when fed on grape and citrus, respectively.

RESUMEN El uso de simbiontes genéticamente modificados es una nueva estrategia para el control de enfermedades transmitidas por insectos mediante la reducción de la competencia vectorial. Esta estrategia de control por medio de simbiontes esta siendo desarrollada para reducir la diseminación de Xylella fastidiosa por Homalodisca vitripennis (Germar) [anteriormente conocido como Homalodisca coagulate (Say)] (Hemiptera: Cicadellidae). En este estudio, el destino de una bacteria simbiótica genéticamente modificada fue evaluada cuando su hospedero, la chicharrita H. vitripennis, SE mantuvo en vid y cítrico. PCR en tiempo real con sondas TaqMan fueron usadas para detectar y cuantificar células bacterianas establecidas en el cibario y precibario del cicadélido al final de 0,2, 4, 9, y 12 días después de la adquisición. El titulo de S1Axd SE redujo drásticamente para el segundo día pos-adquisición; a pesar de ello las chicharritas mantuvieron la infección hacia el 12avo día de muestreo en un 45 y 38% cuando SE alimentaron en vid o cítrico respectivamente.
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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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