Home >> Journal of Economic Entomology, Volume 101, Number 4

Development of a Multiplex Method to Discriminate Between Neochrysocharis formosa (Hymenoptera: Eulophidae) Reproductive Modes

Authors: Adachi-Hagimori, Tetsuya; Miura, Kazuki

Source: Journal of Economic Entomology, Volume 101, Number 4, August 2008 , pp. 1510-1514(5)

Publisher: Entomological Society of America

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Abstract:

A multiplex polymerase chain reaction (PCR) method was applied to differentiate thelytokous and arrhenotokous strains of Neochrysocharis formosa (Westwood). Alignment of strain first internal transcribed spacer regions revealed high nucleotide variability and the strain-specific primer sequence used. Strains were easily differentiated after gel electrophoresis of multiplex PCR products because arrhenotokous specimens produced a 500-bp fragment as well as the 800-bp fragment common to both strains. This method successfully distinguished N. formosa strains regardless of collection site across Japan; thus, it is probably suitable for similar applications in Turkey, Italy, and elsewhere.

Keywords: Neochrysocharis formosa; Liriomyza trifolii; Liriomyza sativae; biological agent; multiplex polymerase chain reaction

Document Type: Short communication

Publication date: 2008-08-01

More about this publication?
  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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