Authors: Kim, Young Ho; Kang, Jae Soon; Kim, Ju Il; Kwon, Min; Lee, Seunghwan; Cho, Hyun Sok; Lee, Si Hyeock

Source: Journal of Economic Entomology, Volume 101, Number 4, August 2008 , pp. 1134-1139(6)

Publisher: Entomological Society of America

Abstract:

We investigated the effects of a diamondback moth-resistant Chinese cabbage (Brassica campestris subsp. napus variety pekinensis Makino), expressing the insecticidal protein Cry1A(c) toxin derived from Bacillus thuringiensis, on the nontarget herbivore Mamestra brassicae (L.)(Lepidoptera: Noctuidae) and its parasitoid wasp Microplitis mediator (Haliday) (Hymenoptera: Braconidae). A decreased survival rate at neonate stage was observed in M. brassicae when reared on Bt cabbage, although overall development was not significantly affected. According to enzyme-linked immunosorbent assay test using Cry1A(c) antibody, the Cry toxin was only detected in the alimentary canal, not in the hemolymph or remaining body parts of M. brassicae, indicating that the ingested Cry toxin is neither distributed inside the body nor transferred through the trophic level. As expected, no Cry toxin was found in the larvae and cocoons of M. mediator. In addition, no significant changes were observed in the parasitization rate, larval period, pupal period, cocoon weight, or adult emergence rate when M. mediator wasps were reared on the M. brassicae larvae fed with transgenic Chinese cabbage. In summary, no direct or indirect adverse effects of transgenic Chinese cabbage on the two nontarget insect species were observed, suggestive of low risk in herbivore-parasitoid food chain.

Keywords: Brassica campestris subsp. napus variety pekinensi; Mamestra brassicae; Microplitis mediator; Bt transgenic Chinese cabbage; enzyme-linked immunosorbent assay

Document Type: Research article

Publication date: 2008-08-01

More about this publication?

• Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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