Application of Molecular Techniques to Distinguish Liriomyza trifolii from L. sativae (Diptera: Agromyzidae) on Tomato Cultivation in Japan

The full text article is not available for purchase.

The publisher only permits individual articles to be downloaded by subscribers.


A molecular method is applied for differentiating the morphologically related leafminers Liriomyza trifolli and L. sativae on tomato cultivation. The method requires multiplex polymerase chain reaction (PCR) amplification of a region of mitochondrial cytochrome oxidase DNA using multiprimer sets. The method divides the mitochondrial fragment of L. trifolli into two fragments and L. sativae into three fragments. It is faster, less costly, and easier than random amplified polymorphic DNA-PCR, PCR-restriction fragment-length polymorphism, and DNA sequencing and more sensitive than the enzyme electrophoresis method, which are other ways to differentiate these two species. We applied the method to samples from populations of another place, sex, and stage and obtained the same results.

Keywords: Liromyza sativae; Liromyza trifolli; mitochondrial cytochrome oxidase; multiplex polymerase chain reaction

Document Type: Research Article

Publication date: June 1, 2004

More about this publication?
Related content



Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more