Authors: Schneider, Marcela Inés; Smagghe, Guy; Gobbi, Antonio; Viñuela, Elisa

Source: Journal of Economic Entomology, Volume 96, Number 4, August 2003 , pp. 1054-1065(12)

Publisher: Entomological Society of America

Abstract:

Susceptibility of the lepidopteran parasitoid Hyposoter didymator (Thunberg) to seven modern insecticides, azadirachtin, diflubenzuron, halofenozide, methoxyfenozide, pyriproxyfen, tebufenozide, and spinosad, was tested in the laboratory. Pupae were exposed to different doses of each compound by direct topical application. At the field recommended doses, methoxyfenozide and tebufenozide had no effect on H. didymator. Halofenozide had a low effect on both adult emergence and adult survival but the progeny size and parasitism capacity were not affected. Diflubenzuron was moderately toxic to the parasitoid, while azadirachtin, pyriproxyfen and spinosad were very toxic, affecting all its life parameters. In the pyriproxyfen and spinosad treatments, no progeny was obtained. As a second approach of this study, we determined the rate of penetration through the pupal cocoon and absorption in the parasitoid body as pharmacokinetic parameters important for toxicity. Most of the radioactivity was retained in the silken cocoon, indicating a low accumulation in the parasitoid body. Among all compounds tested, diflubenzuron exhibited the highest absorption in the parasitoid body, followed by pyriproxyfen. For halofenozide, methoxyfenozide and tebufenozide, low absorption (<2%) was found. In addition, we tested for the presence of molting hormone receptors in Hyposoter tissues using a monoclonal antibody 9B9. Our data suggest that the use of diflubenzuron azadirachtin, pyriproxyfen, halofenozide, and spinosad in combination with H. didymator in integrated pest management (IPM) programs should be carefully evaluated. Methoxyfenozide and tebufenozide could be considered safe for this parasitoid.

RESUMEN Se ha evaluado en laboratorio la susceptibilidad del parasitoide de lepidópteros, Hyposoter didymator (Thunberg) hacia siete insecticidas de nueva generación: azadiractina, diflubenzurón, halofenocida, metoxifenocida, piriproxifén, tebufenocida y spinosad, aplicados tópicamente a las pupas. Metoxifenocida y tebufenocida, a las dosis recomendadas en campo, fueron inocuos para H. didymator. Halofenocida tuvo un efecto mínimo en la emergencia y supervivencia de adultos y no modificó su capacidad benéfica. Diflubenzurón fue moderadamente tóxico para el parasitoide, mientras que azadiractina, piriproxifén y spinosad fueron muy perjudiciales, afectando todos sus parámetros de vida. En los tratamientos con spinosad y piriproxifén no se obtuvo progenie. También se determinaron las tasas de penetración de los insecticidas a través del capullo de la pupa y la absorción en el cuerpo del parasitoide, pues ambos parámetros farmacocinéticos son importantes para la toxicidad. La mayoría de la radioactividad se retuvo en el capullo de seda, indicando una baja acumulación en el cuerpo del parasitoide. Entre todos los compuestos evaluados, diflubenzurón mostró la absorción más elevada en el cuerpo del parasitoide seguido por piriproxifén. Para halofenocida, metoxifenocida y tebufenocida la absorción fue inferior al 2%. Además, evaluamos la presencia de receptores de la hormona de la muda en tejidos de Hyposoter usando el anticuerpo monoclonal 9B9. Nuestros datos sugieren que el empleo conjunto del diflubenzurón, la azadiractina, el piriproxifén, el halofenocida y el spinosad con H. didymator en programas de manejo integrado de plagas, deberá ser evaluado cuidadosamente. Metoxifenocida y tebufenocida podrían ser considerados no dañinos para este parasitoide.

Keywords: Hyposoter didymator; parasitoid; pupae; IGRs; naturalyte; cocoon penetration

Document Type: Research article

DOI: http://dx.doi.org/10.1603/0022-0493-96.4.1054

Publication date: 2003-08-01

More about this publication?

• Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
• Editorial Board
• Submit a Paper
• Subscribe to this Title
• Information for Advertisers
• Visit this journal's homepage
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Entomology
• By this author: Schneider, Marcela Inés ;  Smagghe, Guy ;  Gobbi, Antonio ;  Viñuela, Elisa

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers