If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Long-Term Selection for Resistance to Bacillus thuringiensis Cry1Ac Endotoxin in a Minnesota Population of European Corn Borer (Lepidoptera: Crambidae)

$28.00 plus tax (Refund Policy)

Buy Article:

Abstract:

Transgenic corn, expressing the insecticidal δ-endotoxin of Bacillus thuringiensis Berliner, provides high levels of control of some lepidopteran pests, particularly the European corn borer, Ostrinia nubilalis (Hübner). However, resistance to B. thuringiensis has been documented recently in laboratory colonies of agronomically important Lepidoptera, including O. nubilalis. For the past 4 yr, we have selected for Cry1Ac resistance in a population of O. nubilalis from southeastern Minnesota. Increasing resistance to B. thuringiensis was noted after only 8 generations of selection, with a peak at 162-fold resistance, based on comparisons of LC50s to a nonselected parental strain. This resistance was found to decrease at the same rate in the absence of B. thuringiensis selection, with one selected colony becoming nearly as sensitive to the Cry1Ac toxin as the nonselected colony after 9 generations without exposure to B. thuringiensis. The most resistant of the colonies, S-I, was only marginally cross-resistant to Cry1Ab, yet another selected colony, S-IV, did demonstrate a 16-fold cross-resistance. In addition, larvae from the S-IV colony had significantly greater weight gain when feeding on diet incorporated with B. thuringiensis-transgenic corn than did larvae from the nonselected parental colony. These findings emphasize the need for careful deployment of B. thuringiensis corn to preserve this effective pest management technology.

Keywords: Bacillus thuringiensis; Ostrinia nubilalis; cross-resistance; resistance management; selection

Document Type: Research Article

Publication date: October 1, 1999

More about this publication?
  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
  • Editorial Board
  • Submit a Paper
  • Subscribe to this Title
  • Information for Advertisers
  • Visit this journal's homepage
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more