Differences in Cellular Abnormalities Induced by the Probing Behaviors of Empoasca kraemeri (Homoptera: Cicadellidae) on Tolerant and Susceptible Common Beans
Authors: SERRANO, MIGUEL S.; BACKUS, ELAINE A.
Source: Journal of Economic Entomology, Volume 91, Number 6, December 1998 , pp. 1481-1491(11)
Publisher: Entomological Society of America
Abstract:Cellular abnormalities induced by the feeding behavior of Empoasca kraemeri Ross & Moore were studied in 2 tolerant lines ('EMP 84' and 'EMP 392'), an intermediate ('Porrillo sintetico') and a susceptible ('Calima') cultivar of Phaseolus vulgaris L. Groups of two 3- to 4-d-old Female leafhoppers were placed for 3 h in l clip cage per plant, each covering 1cm2 of fully expanded primary laeves, 1 5 dafter planting. Leaf tissues were fixed 48 h after this access period and prepared for light and confocal microscopy. Damage to the leaf lamina in all genotypes included cell emptying and breakage of cell walls, as well as enlarged intercellular spaces in the palisade and spongy parenchyma. In the main vascular bundle, hypertrophied parenchyma cells plus cells with enlarged nuclei and stained nucleoli were observed. In general, cellular damage was less severe in the tolerant lines than in the susceptible genotypes, although more so for EMP 84 than for EMP 392. Morphometry of autofluorescent tracheary elements showed what seems to be a compensatory response to leafhopper attack in the tolerant lines. Although control leaves from tolerant lines had fewer tracheary elements per vascular bundle after leafhopper feeding, EMP 84 had more tracheary elements in damaged tissues. Both tolerant genotypes had tracheary elements with larger internal radii, which increased their estimated relative flow rates. The implications of cellular damage for the onset of hopperburn, in bean as well as other host plants such as alfalfa and potato, and the mechanisms of tolerance are discussed.
Document Type: Research Article
Publication date: 1998-12-01
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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