Using an F2 Screen to Search for Resistance Alleles to Bacillus thuringiensis Toxin in European Corn Borer (Lepidoptera: Crambidae)
Authors: ANDOW, D. A.; ALSTAD, D. N.; PANG, Y.H.; BOLIN, P. C.; HUTCHISON, W. D.
Source: Journal of Economic Entomology, Volume 91, Number 3, June 1998 , pp. 579-584(6)
Publisher: Entomological Society of America
Abstract:We present an application of an F2 screening method for recovering and estimating the frequencies of rare alleles that confer insect resistance to a transgenic corn variety producing Bacillus thuringiensis Berliner crystal protein toxin (Bt corn). Based on a sample of 91 female Ostrinia nubilalis (Hübner) we show with 95% confidence that the frequency of B. thuringiensis resistance alleles from a wild Minnesota population is <0.013. This is an upper limit to the estimated allele frequency and does not provide clear evidence that 1 of the assumptions of the refuge plus high-dose strategy will or will not be met. With additional sampling, a more precise estimate of resistance allele frequency could be obtained that would clearly support or refute 1 of the assumptions of the refuge plus high-dose strategy. Variable costs of the screening method were 19.70per female line, but these could be reduced by improved collecting, rearing, and handling methods.
Document Type: Research Article
Publication date: 1998-06-01
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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