Evaluation of Natural and Engineered Resistance Mechanisms in Solanum tuberosum for Resistance to Phthorimaea operculella (Lepidoptera: Gelechiidae)

Authors: WESTEDT, A. L.; DOUCHES, D. S.; PETT, W.; GRAFIUS, E. J.

Source: Journal of Economic Entomology, Volume 91, Number 2, April 1998 , pp. 552-556(5)

Publisher: Entomological Society of America

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Abstract:

Potato tuber moth, Phthorimaea operculella Zeller, is a highly destructive pest of cultivated potato, Solanum tuberosum L., and is responsible for damage to both leaf and tuber tissues. Host plant resistance is a central component to developing an integrated pest management program to control potato tuber moth. This research tested the efficacy of a codon-modified CryV-Bacilius thuringiensis (CryV-Bt) gene constitutively expressed in potato and the combined effect of CryV-Bt expression with natural host plant resistance mechanisms in potato. 'Lemhi Russet' and 2 lines with host plant resistance mechanisms, USDA8380-1 (leaf leptines) and L235-4 (glandular trichomes), along with the CryV-Bt-transgenic lines of each of these 3 genotypes were examined. Detached leaf bioassays were conducted to examine control of potato tuber moth. Nontransformed Lemhi Russet and L235-4 were susceptible to potato tuber moth, while 54%potato tuber moth mortality was found when first instar larvae fed on USDA8380-1Ieaves. High levels of expression occurred in the CryV-Bt transgenic lines, with up to 96% potato tuber moth mortality. These transgenic lines provide a germplasm base to examine combined insect-resistance mechanisms as a means to achieve durable host plant resistance.

Document Type: Research Article

Publication date: April 1, 1998

More about this publication?
  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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