Evaluation of Miniature and High. Volume Bioassays for Screening Insecticides
Authors: JANSSON, RICHARD K.; ROSS HALLIDAY, W.; ARGENTINE, JOSEPH A.
Source: Journal of Economic Entomology, Volume 90, Number 6, December 1997 , pp. 1500-1507(8)
Publisher: Entomological Society of America
Abstract:Three methods for screening insecticides and acaricides were evaluated against select arthropod targets: tobacco budworm, Heliothis cirescens (F.), beet armyworm, Spodoptera exigua (Hübner), Colorado potato beetle, Leptinotarsa decemlineata (Say), and the twospotted spider mite, Tetranychus urticae Koch. Methods varied in amounts of test solutions delivered and included a moderate pressure rotating spray tower that sprayed 50 ml of solution to runoff on 3 plant species, and 2 miniature volume assays; a low pressure, airbrush applicator that delivered 1 ml to both surfaces of individual leaves; and an agar-based artificial diet assay (Lepidoptera only) that delivered small aliquots (50 μl) of test solutions to the surface of the diet. Bioassays were compared using 2 avermectin insecticides, abamectin and emamectin benzoate, 2 pyre-throid insecticides, fenvalerate and lambda-cyhalothrin, 2 organophosphorous insecticides, mevinphos and trichlorfon, and 1 ecdysone agonist, tebufenozide. All 3 methods were comparable at estimating the sensitivity of arthropods to these compounds. Differences in lethal concentration values among assays were species specific. Variation among assays was more apparent with less potent compounds (e.g., organophosphorous compounds and tebufenozide) compared with more potent compounds (pyrethroids and avermectins) for H. virescens, whereas in S. exiglla and T.urticae, lethal concentration values were comparable among bioassay types for most compounds tested. These data indicate that low volume bioassays were comparable to high volume bioassays at detecting and estimating insecticidal activity against a select panel of arthropod pests. The potential of miniature volume bioassays for use in insecticide discovery programs is discussed, with particular reference to screening programs that rely Oil sample collections that are available in limited quantities, such as natural product and combinatorial chemistry sources.
Document Type: Research article
Publication date: 1997-12-01
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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