Glass-Vial Bioassay To Estimate Insecticide Resistance in Adult Tarnished Plant Bugs (Heteroptera: Miridae)

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A glass-vial bioassay was developed for use in estimating resistance to insecticides in adult tarnished plant bugs, Lygus lineolaris (Palisot de Beauvois). When laboratory reared insects of known age are used, the bioassay procedure requires that adults are not older than 10 d. Testing each sex separately was not required, and each glass-vial (treated on its inside with an insecticide) received a piece of green bean, Phaseolus vulgaris L., as food for the adults tested. Two or 3 adults were placed in each vial; mortality was determined after 24 h of exposure in the vial. The bioassay was used to determine insecticide resistance in 3 groups of tarnished plant bugs to 10 different insecticides. Significant differences in resistance among adults were found from the 3 groups with most of the insecticides. Data obtained with this glass-vial bioassay can be compared with data obtained from bioassays that use plant bugs collected from cotton, Gossypium hirsutum L., fields in which control with insecticides has been inadequate.

Document Type: Research Article

Publication date: October 1, 1996

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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