Genotypic Variation Among Wild Isolates of Douglas-Fir Tussock Moth (Lepidoptera: Lyntantriidae) Nuclear Polyhedrosis Virus

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Larvae of the Douglas-fir tussock moth, Orgyia pseudotsugata (McDunnough), were reared from egg masses collected at 10 locations in 6 geographic regions of British Columbia. DNA of O. pseudotsugata nuclear polyhedrosis virus (OpMNPV) extracted from these larvae and from commercial OpMNPV viral-insecticide preparations (Virtuss ,and TMBioControl-1) were subjected to restriction endonuclease (REN) analysis with PstI and SaII. Southern blot analyses of the REN cleavage products revealed polymorphisms in 54.5% of samples digested with PstI and 43.3% of samples digested with SaII. Five PstI genotypes and 2 SalI genotypes were detected. Each of the five Pst/I REN patterns demonstrated either one or the other of the SalI REN patterns, creating 10 possible combined genotypes, 8 of which were actually detected. Hare and common genotypes were mapped to each of the 6 geographic regions.

Document Type: Research Article

Publication date: June 1, 1996

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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