Toxicological and Biochemical Characterization of Insecticide Resistance in a Field-Collected Strain of Blattella germanica (Dictyoptera: Blattellidae)

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Abstract:

A combination of insecticidal, biochemical, and electrophoretic assays were conducted on adult male and 1st instars of a field-collected strain of German cockroach, Blattella germanica (L.) (Muncie'86), to characterize its insecticide resistance. In topical applications to adult males, 11 technical grade insecticides were tested over 3 yr. These included representatives of 4 chemical classes: (1) pyrethroids (5 compounds), (2) oregano phosphates (3 compounds), (3) carbamates (2 compounds), and (4) cyclodienes (1 compound). The highest levels of resistance were to chlordane and bendiocarb with a resistance ratio at 95% response of 21.9 and 15.7, respectively. To examine the stability of resistance, the responses of adult males to chlorpyrifos and cypermethrin were tested repeatedly over 3 yr and results were compared by chi-square analysis. Resistance levels showed significant yearly variation in each of the 50 and 95% resistance ratios, but only significant insecticidal variation at the 50% level. Treatment with piperonyl butoxide before insecticides restored susceptibility to only 1 of 4 insecticides tested on adult males, suggesting that a monooxygenase resistance mechanism coexists with other mechanisms in this strain. Biochemical assays confirmed that this strain possessed both elevated levels of cytochrome P450 monooxygenase (in the adult male stage) and est erase activity (in 1st instars), compared with a standard susceptible strain of German cockroach. Native est erases from 1st instars in the field-collected strain differed electrophoretically from those in the susceptible strain.

Document Type: Research Article

Publication date: April 1, 1996

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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