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Alternative Bioassay Technique To Measure Activity of Cry III Proteins of Bacillus thuringiensis

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Abstract:

Problems in reproducibility of results of the standard dip-leaf bioassay using the subspecies tenebrionis of Bacillus thuringiensis Berliner on Leptinotarsa texana Schaeffer, indicated the necessity for development of an alternative technique. The alternative technique is based on the inverse relationship of dose and weight gain of starved L. texana 3rd instars. Individuals avidly ingested a 2-l B. t. tenebrionis sucrose droplet and were reared on natural diet for 48 h. Linear regression analysis estimated ED50s (effective dose for 50% response) compared with weight gain of untreated individuals. Correlation coefficients >0.9 were always obtained. Reproducibility between bioassays was very high, despite the low number of treated individuals (10 per dose). Bioassay time was reduced from 4 d to only 48 h.

Document Type: Research Article

Publication date: December 1, 1995

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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