Phthorimaea operculella (Lepidoptera: Gelechiidae) Resistance in Potato by Expression of the Bacillus thuringiensis CryIA(b) Insecticidal Cry~tal Protein

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Abstract:

The potato tuberworm, Phthorimaeaoperculella (Zeller), is a primary lepidop-teran pest of potato and is susceptible to the CryIA(b) insecticidal crystal protein of Bacillus thuringiensis.We introduced T-DNAs containing the TR2' promoter fused to the native tnmcated cryIA(b) gene (bt884) into the potato lines 'Kennebec' and 'Bintje' and T-DNAs containing the TR2' promoter fused to a modified truncated cryIA(b) gene (cryIAb6) into Kennebec and 'Yesmina'.A first selection of potato tuberworm resistant lines was made on the basis of enzyme-linked immunoassays, Manduca sexta (L.) leaf bioassays, and potato tuberworm leaf and tuber bioassays. In greenhouse trials, the selected potato lines carrying the bt884 gene showed significantly less potato tuberworm leaf damage than the control lines. However,the number and size of leaf tunnels increased with time. Similarly,the Yesminalines expressing the crylAb6 gene gave significantly less potato tuberworm leaf damage than the control line, which did not increase with time. In storage bioassays in which tubers were infested with neonate larvae and stored for ≍2 mo, all transgenic lines gave almost 100% potato tuberworm control. Tubers of crylAb6 potato lines stored for up to 7 mo at 4 or 25 also resulted in 100% mortality.

Document Type: Research Article

Publication date: October 1, 1995

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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