Biochemical Properties of Esterases in Pyrethroid-Resistant and -Susceptihle Strains of the Horn Fly (Diptera: Muscidae)
Authors: XU, GANG; BULL, DON L.
Source: Journal of Economic Entomology, Volume 88, Number 5, October 1995 , pp. 1186-1191(6)
Publisher: Entomological Society of America
Abstract:In vitro tests using whole body homogenate of pyrethroid-resistant and -susceptible horn flies, Haematobia irritans L., indicated that nonspecific esterase activity against the substrate x-naphthyl acetate was 1.53-fold greater in the resistant strain. Most of the nonspecific esterase activity in both strains was attributable to membrane-bound acetylcholinesterase (AChE). Carboxylesterases(CarE) were associated primarily with the cytosolic fraction of homogenates, and resistant flies had the greatest titers of these enzymes. Permethrin esterase, assayed in the cytosol with the substrate trans-permethrin, was most active in the resistant strain. Several isozymes of CarE and AChE were resolved by nondenaturing polyacrylamide gel electrophoresis, and differential staining of the gels revealed distinct differences between strains in titers of some of the isozymes.
Document Type: Research Article
Publication date: October 1, 1995
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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