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Simple Method for Monitoring Dispersal of Heliothi (Lepidoptera: Noctuidae) Backcross Sterility Genes

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Release of sterile backcross males from [Heliothis subfliexa (F.) X H. virescens (Guenée) females X H. virescens males I has been suggested as a means of reducing populations of H. virescens. A simple method is needed to measure the persistence or dispersal of the backcross sterility genetic factors. Backcross male sterility is transmitted via maternal inheritance. The mitochondrial DNA (mtDNA) also is inherited maternally; therefore, the mtDNA in the backcross is from H. subflexa. Several regions of the mtDNA were amplified using polymerase chain reaction (PCR), then were cut with restriction enzymes to generate restriction fragment length polymorphisms (RFLP). Differences between H. virescens and backcross mtDNA were observed for 16S (ribosomal DNA (rDNA)), 12S-16S rDNA, and the COI-COII (cytochrome oxidase) regions. This is attributed to species-specific differences between H. virescens mtDNA and H. subflexa mtDNA carried by the backcross. Therefore, mtDNA provides a convenient marker for tracking the progress of backcross sterility. Developmental stages from eggs through adults are suitable for use.

Document Type: Research Article

Publication date: June 1, 1994

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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