Analysis of Gut Contents and Feeding Behavior of Tipulid Larvae (Diptera: Tipulidae) Using a New Root-Staining Technique
Authors: VLUG, H. J.; HARREWIJN, P.
Source: Journal of Economic Entomology, Volume 87, Number 1, February 1994 , pp. 101-102(2)
Publisher: Entomological Society of America
Abstract:To determine whether first-instar Tipula oleracea L. preferentially feed on either grass roots or leaves, we developed a differential staining technique for grass roots. A 0.15% aqueous solution of trypan-blue stained the root-system of ryegrass sufficiently to identify root tissue in the gut system of leatherjackets. It did not stain leaf tissue of the same plants. Using stained grass plants, it was shown that young larvae of T. oleracea in grasslands preferentially feed on leaf tissue and not on root tissue as previously assumed.
Document Type: Research Article
Publication date: February 1994
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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