Acetylcholinesterase from the Horn Fly (Diptera: Muscidae): Distribution and Purification

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Abstract:

Acetylcholinesterase (AChE) in newly emerged adults of the horn fly, Haematobia irritans (L.), was distributed mostly in the head (72.9%), less in the thorax (24.4%), and least in the abdomen (2.7%). The major portion of total AChE was membrane-bound and associated primarily with the microsomes. The enzyme was solubilized with phosphate buffer containing 0.5% (vol/vol) of Triton X-lO0 and purified with affinity chromatography. The extent of purification was about 217-fold for the membranous fraction and 329-fold for the soluble fraction of whole fly homogenates with recoveries of 55% and 79%, respectively. The extent of purification reached 600- and 784-fold for the membranous and the soluble fractions, respectively, in the collections of eluents with peaked activity of AChE. Electrophoresis revealed four bands of AChE activity in the membrane-bound preparation and three bands in the soluble preparation. Silver staining of the electrophoresed gels showed that the major proteins in purified AChE solutions corresponded with AChE bands.

Document Type: Research Article

Publication date: February 1, 1994

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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