Genome Size and Endopolyploidy in Pyrethroid-Resistant and Susceptible Strains of Heliothis virescens (Lepidoptera: Noctuidae)

Authors: Taylor, Martin; Zawadzki, Jerome; Black, Bruce; Kreitman, Martin

Source: Journal of Economic Entomology, Volume 86, Number 4, August 1993 , pp. 1030-1034(5)

Publisher: Entomological Society of America

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Abstract:

Heliothis virescens (F.) males have a haploid genome size of 403.6 (2.3 ± SD) million nucleotide pairs (Mnp), which is very close to that reported for tissues of other Lepidoptera (456-485 Mnp). Octaploid cell types were present in various abundances in all tissues examined, and thus may derive from a common tissue such as epithelial tissue. Extremely (i.e., 104- to l05-fold) polyploid nurse or silk gland cells are known in other Lepidoptera. However, attempts to analyze salivary glands of H. virescens, which might also be highly polyploid, were not successful. Cells of higher ploidy were not found in any of the tissues examined. Incomplete duplication of higher ploidy cells was found in both fat body and Malpighian tubules. The cells of the Malpighian tubules were found to be inflated in DNA content relative to other tissues, for reasons unknown. Nuclei were analyzed from larvae of both a pyrethroid-resistant and a susceptible strain of H. virescens. Differences in DNA contents between strains were sex specific and were not consistent with the hypothesis that large-scale DNA amplification contributes to pyrethroid resistance in the RR strain.

Document Type: Research Article

Publication date: August 1, 1993

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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