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Comparison of Two Bioassay Techniques for Larvae of Helicoverpa spp. (Lepidoptera: Noctuidae)

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A method to bioassay contact insecticides against first instars of Helicoverpa Armigera (Hübner) and Helicoverpa punctigera (Wallengren) is described. Just before hatching, fertile eggs were sprayed with proprietary emulsifiable concentrate formulations of fenvalerate, endosulfan, and methyl parathion. Emerging first instars were exposed to insecticide from contact with the egg shell and its sprayed surroundings. Bioassays showed a significant relationship between concentration (AI) and mortality of first instar. The test method showed differences in intrinsic insecticide susceptibility of H. armigera and H. punctigera and distinguished between susceptible and resistant H. armigera. The validity of the first-instar bioassay technique was confirmed by comparison with established techniques. The relevance of this bioassay method to resistance monitoring for the Australian Helicoverpa resistance management strategy is discussed.

Document Type: Research Article

Publication date: April 1, 1993

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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