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Maintenance of Infectivity and Virulence of Nuclear Polyhedrosis Viruses During Serial Passage in Noctuid (Lepidoptera: Noctuidae) Cell Lines

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Abstract:

Nuclear polyhedrosis viruses isolated from the cabbage looper, Tnchoplusia ni (Hubner) (TnMNPV), Rachiplusia ou (GuemJe)(RoMNPV),and a plaque purified variant of Autographa califarnica (Speyer) (AcMNPV6R)were serially passaged numerous times in larvae of the cabbage looper and the beet armyworm, Spodoptera exigua (Hubner). These viruses were then serially passaged in the IPLB-SF-21AE cell line from the fall armyworm, Spodoptera frugtperda (]. E. Smith), and in the TNFH-368 cell line from T. ni in standard media and in media fortified with the addition of liposomal material. All of the viruses, after being passaged in these two cell lines, were bioassayed in neonate T. ni to evaluate the effect of the additional sterol and lipids in the growth media on the viruses. For each of the viruses produced in the TNFH-368 cell line with the addition of liposomes, the fifth passage virus was more virulent than the fifth passage virus in this cell line without the supplement. In addition there was no significant loss in the virulence of the fifth passage virus from that of the first passage virus when the cell growth medium was supplemented with liposomal material. It appeared that the addition of liposomal material was not as necessary for the production of these viruses when cultured in the IPLB-SF-21AE cell line as when cultured in the TNFH-368 cell line. The growth medium used for the IPLB-SF-21AEline is balanced so that the cells can utilize the exogenous sterols and lipids provided by the serum supplement in the standard medium in such a manner that the virulence of the enveloped viruses produced in this cell line did not deteriorate in the absence of liposomes.

Document Type: Research Article

Publication date: April 1, 1991

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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