Morphology and Cytochemistry of Hemocytes and Analysis of Hemolymph from Melanoplus sanguinipes (Orthoptera: Acrididae)
Authors: MIRANPURI, GURWATTAN S.; BIDOCHKA, MICHAEL J.; TOURIANS, GEORGE KHACHA G.
Source: Journal of Economic Entomology, Volume 84, Number 2, April 1991 , pp. 371-378(8)
Publisher: Entomological Society of America
Abstract:The hemolymph and the hemocytes from the migratory grasshopper, Melanu/ us sanguintpes (F.), were characterized. Ten-d-old male grasshoppers had similar hemolymph osmolality (375 mOsm/kg) and pH (7.6) compared with female grasshoppers. However, females had twice the concentration of hemolymph protein and carbohydrate compared with males. Furthermore, SDS-PAGE separation of hemolymph proteins showed sexual differences in the number and quantity based on the intensity of staining of protein species. Various buffers were evaluated for effectiveness in maintaining hemocyte stability. In some buffers, the hemocytes lysed, clumped together, or were osmotically unstable, A physiologicallyisotonic saline buffer, which maintained hemocyte integrity for up to 30 min, was formulated. This buffer C'onsistedof 1.5mM K.HPO" 8.0 mM NaH.PO" 0.9 mM CaCl., 2.7 mM KCl, 0.5 mM MgCl., and 0.3 mM NaC\. Two major types of hemocytes were observed in M. sanguinipes: granulocytes comprised :::::95% of the total hemocyte population and plasmatocytes constituted the remainder. Scanning electron and phase-contrast microscopy revealed that granulocytes were round in shape and had a central nucleus and a granular cytoplasm. Plasmatocytes had a larger nucleus than that of granulocytes and the cells were spindle-shaped. The two types of hemocytes could not be distinguished from one another when the hemocytes were exposed to enzymatic or nonenzymatic cytochemical stains. The granulocytes of grasshoppers that had been previously injected with conidia of the entomopathogenic fungus Beauveria bassiana were observed to lyse and exude mucopolysaccharide strands to which conidia, melanized particles, and other granulocytes adhere.
Document Type: Research Article
Publication date: April 1, 1991
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
- Editorial Board
- Submit a Paper
- Subscribe to this Title
- Information for Advertisers
- Visit this journal's homepage
- ingentaconnect is not responsible for the content or availability of external websites