Techniques for Studying Pollen Adhering to Nectar-Feeding Corn Earworm (Lepidoptera: Noctuidae) Moths Using Scanning Electron Microscopy
Authors: BRYANT, V. M.; PENDLETON, M.; MURRY, R. E.; LINGREN, P. D.; RAULSTON, J. R.
Source: Journal of Economic Entomology, Volume 84, Number 1, February 1991 , pp. 237-240(4)
Publisher: Entomological Society of America
Abstract:Corn earworm, Helicoverpa zea (Boddie), male moths (399) were collected from sexpheromone traps during spring and late summer for determination and identification of attached pollen. Comparisons made between dissecting light and scanning electron microscopy (SEM) show that direct mounting and viewing by SEM is a much more reliable technique for locating and separating pollen from other debris and for identifying pollen types. Red fingernail polish provides good conductive surfaces and better bonding for mounting specimens to SEM stubs than does silver paint and several other mounting media. Viewing all moth specimens by SEM is more costly, but added cost is partially offset by time saved in eliminating the preliminary examination by dissecting microscopes ('" 15 min per specimen). About3.5h are required for preparation, analysis,and reporting result from individual moth specimens when using only SEM.
Document Type: Research Article
Publication date: February 1991
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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