Laboratory Bioassay for Resistance in Corn to Fall Armyworm (Lepidoptera: Noctuidae) and Southwestern Corn Borer (Lepidoptera: Pyralidae)

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Inbred lines of corn, Zea mays L., were evaluated for resistance to leaf feeding by the fall armyworm, Spodoptera fTugipeTda 0. E. Smith), and southwestern corn borer, Diatraea grandiosella Dyar, in field and laboratory experiments. For the laboratory bioassays, diets were prepared from lyophilized whorl tissue of field grown plants of resistant and susceptible corn inbred lines. To prepare the diets, 11 g lyophilized tissue was stirred into a mixture of 250 ml distilled water, 2 g agar, 12.5 mg gentamicin sulfate, 132 mg sorbic, and 528 mg ascorbic acid that had been heated to 82┬░Cand poured into 30-ml plastic cups. Cups were infested with two neonate larvae each. Fall armyworm larvae reared for 10 d on diets containing tissue of resistant inbred lines weighed 60% less than those reared on diets containing susceptible inbred tissue. Southwestern corn borer larvae reared for 14 d on whorl tissue from resistant inbred lines weighed 50% less than those reared on susceptible tissue. The laboratory bioassay satisfactorily differentiated among resistant and susceptible corn inbreds.

Document Type: Research Article

Publication date: August 1, 1990

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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