Analysis of the Haemolymph of Plutella xylostella (Lepidoptera: Noctuidae) and Subsequent Development of a Physiological Saline

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Abstract:

The haemolymph of final instars ofPlutella xylostella (L.) was analyzed to determine the concentrations of principal ions, osmolarity, pH, amino acids, proteins, and carbohydrates. Results were used to formulate a physiologically isotonic saline for this important pest of cruciferous crops; the saline was composed of 10 mM tripotassium citrate, 15 mM MgCI2, 2 mM CaCI2., 3 mM MgSO4 7 mM Na2 ß-glycerophosphate, and 211 mM glucose. Buffering was achieved with a 10-mM mixture of KH2PO4 and K2HPO4. This medium promoted overall survival of dissected P. xylostella better than any other lepidopteran salines used and was particularly useful as a bathing medium for long-term neurophysiological investigations.

Document Type: Research Article

Publication date: August 1, 1989

More about this publication?
  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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