Analysis of the Haemolymph of Plutella xylostella (Lepidoptera: Noctuidae) and Subsequent Development of a Physiological Saline

The full text article is not available for purchase.

The publisher only permits individual articles to be downloaded by subscribers.

Abstract:

The haemolymph of final instars ofPlutella xylostella (L.) was analyzed to determine the concentrations of principal ions, osmolarity, pH, amino acids, proteins, and carbohydrates. Results were used to formulate a physiologically isotonic saline for this important pest of cruciferous crops; the saline was composed of 10 mM tripotassium citrate, 15 mM MgCI2, 2 mM CaCI2., 3 mM MgSO4 7 mM Na2 ß-glycerophosphate, and 211 mM glucose. Buffering was achieved with a 10-mM mixture of KH2PO4 and K2HPO4. This medium promoted overall survival of dissected P. xylostella better than any other lepidopteran salines used and was particularly useful as a bathing medium for long-term neurophysiological investigations.

Document Type: Research Article

Publication date: August 1, 1989

More about this publication?
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more