Analysis of the Haemolymph of Plutella xylostella (Lepidoptera: Noctuidae) and Subsequent Development of a Physiological Saline

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The haemolymph of final instars ofPlutella xylostella (L.) was analyzed to determine the concentrations of principal ions, osmolarity, pH, amino acids, proteins, and carbohydrates. Results were used to formulate a physiologically isotonic saline for this important pest of cruciferous crops; the saline was composed of 10 mM tripotassium citrate, 15 mM MgCI2, 2 mM CaCI2., 3 mM MgSO4 7 mM Na2 ß-glycerophosphate, and 211 mM glucose. Buffering was achieved with a 10-mM mixture of KH2PO4 and K2HPO4. This medium promoted overall survival of dissected P. xylostella better than any other lepidopteran salines used and was particularly useful as a bathing medium for long-term neurophysiological investigations.

Document Type: Research Article

Publication date: August 1, 1989

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