Biochemical and Immunochemical Properties of HPLC Peak 2, an Ion-Exchange Fraction of Common Cattle Grub (Diptera: Oestridae)

Authors: SCHWINGHAMMER, K. A.; PRUETT, J. H.; TEMEYER, K. B.

Source: Journal of Economic Entomology, Volume 81, Number 2, April 1988 , pp. 549-554(6)

Publisher: Entomological Society of America

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Abstract:

Soluble proteins extracted from first instar common cattle grub, Hypoderma lineatum (Villers), can be resolved into four major proteolytic fractions by HPLC ionexchange chromatography. Hypodermin A, hypodermin B, and collagenase are serine proteases contained in three of the four fractions and have been described previously. HPLC peak 2 (P2), another fraction of first instar H. lineatum, was prepared and compared with the three other protease fractions. P2 exhibited tryptic esterase activity (as indicated by hydrolysis of amide and ester substrates of trypsin) and no chymotryptic esterase activity. Although tryptic activity within P2 was inhibited by both soybean and bovine pancreatic trypsin inhibitors, chicken ovomucoid had little inhibitory effect. Ethylenediaminetetraacetic acid (EDTA) and N-α-tosylphenylalanine chloromethyl ketone (TPCK, a chymotrypsin inhibitor) were without effect on P2 tryptic esterase activity, while the trypsin inhibitors benzamidine and N-α-tosyl-L-lysyl chloromethyl ketone (TLCK) were slightly inhibitory. Tryptic esterase activity of P2 was relatively stable at temperatures from 20 to 50 and had pH optimum of 7.0-7.8. Results indicated that hypodermin B and P2 protease activities were more closely related to one another than they were to hypodermin A. lmmunochemical cross-reactivity suggested antigenic similarities between hypodermin B and P2, yet hypodermin A was found to be antigenically distinct.

Document Type: Research Article

Publication date: April 1, 1988

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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