Vinyl Plastic Cage Design for Single-Mating Experiments to Chemosterilize the Stable Fly (Diptera: Muscidae) with Bisazir

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Small cylindrical cages constructed of heavy clear plastic were designed to evaluate the chemosterilant bisazir for control of the stable fly, Stomoxys calcitrans (L.). Fly larvae were mass-reared using artificial media in cloth-covered plastic pans. Developing pupae were recovered from the media using forced air from a portable hair dryer. The pupal immersion technique was used to evaluate a 1% solution of bisazir to sterilize the insects. After sterilization, emerging males and females were placed in plastic cages and fed daily, using small pieces of blood-soaked pads placed on top of the screen. Fertile flies oviposited on the pads after 4 d. The pads were changed daily, and any eggs present were transferred to black filter paper in Petri dishes for 2 d to determine hatching rate. No eggs were laid by females emerging from pupae receiving a 90-min treatment; eggs laid by fertile females mated with treated males did not hatch.

Document Type: Research Article

Publication date: February 1, 1988

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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