An efficient method to infest cotton, Gossypium hirsutum L., plants artificially in the field with tarnished plant bug (TPB), Lygus lineolaris (Palisot de Beauvais), nymphs was developed for host plant resistance studies. A selected number of TPB nymphs are placed in or near the plant terminal with a small artist's brush. Damage was evaluated by plant symptoms (aborted terminals, swollen nodes, and excessive branching of the main stem) and reduction in yield (nymph-infested plots had yield reduction of 22% for the first harvest and 25% for total yield across eight cotton cultivars). Large numbers of nymphs of the same age were provided from an improved rearing technique, developed during this study, that utilized pole beans, Phaseolus vulgaris L., and Romaine lettuce, Lactuca sativa L.
Document Type: Research Article
Publication date: October 1, 1986
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