Improved Feeding Bioassay for the Boll Weevil (Coleoptera: Curculionidae)

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An improved feeding bioassay was developed to assess effects of semiochemicals on feeding by adult boll weevils, Anthonomus grandis grandis Boheman. The bioassay eliminates reliance on cotton squares or preparation of paper-wrapped feeding plugs common to previous methods. Any solvent may be used to deliver semiochemicals without observable adverse effects on the insect's feeding. A series of experiments was conducted to select a preferred media and its surface shape, number of feeding sites (wells) and insects per bioassay dish, and light conditions. The reliability of the method was determined by comparing results generated from it with those from a dipped cotton square assay. Methanolic solutions of quercitrin, rutin, and crude cotton tannin were coated onto cotton squares and also applied to the lens papers in the plate bioassay. Quercitrin was a feeding deterrent in both bioassays while rutin was a feeding stimulant in the plate bioassay. Tannin was neither stimulatory nor deterrent compared with controls in either bioassay.

Document Type: Research Article

Publication date: June 1, 1986

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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