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Laboratory Evaluation of New Ultraviolet Absorbers for Protection of Douglas-fir Tussock Moth (Lepidoptera: Lymantriidae) Baculovirus

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Abstract:

In 1975, the Forest Service (USDA) recommended use of the adjuvant Shade as a UV absorber in spray mixes of TM BioControl-1, the nuclear polyhedrosis virus (NPV) of Orgyia pseudotsugata (McDunnough). Because Shade is no longer available commercially, we evaluated suitable substitutes compatible with the NPV of O. pseudotsugata. Materials tested included two lignosulfonates, a disulfobenzophenone, and two fluorescent whitening agents. Shade was also tested as a reference standard. The materials were evaluated by exposing virus-absorber mixtures to UV radiation from high-intensity mercury sun lamps. We used a new technique that permitted individual handling of samples, on sterile disposable Teflon pads, through various steps of the test procedure. Two of the materials, Tinopal DCS (a stilbene fluorescent whitening agent) and Raymix powder (a lignosulfonate), protected O. pseudotsugata NPV against UV radiation equally or better than did Shade. Both UV absorbers are soluble in cold water, with pH near neutrality.

Document Type: Research Article

Publication date: August 1, 1985

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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