Semiquantitative Bioassay for Levels of Cucurbitacins Using the Banded Cucumber Beetle (Coleoptera: Chrysomelidae)
Authors: Peterson, J. K.; Schalk, J. M.
Source: Journal of Economic Entomology, Volume 78, Number 4, August 1985 , pp. 738-741(4)
Publisher: Entomological Society of America
Abstract:Various concentrations of semipurified cucurbitacin solutions were applied to chromatography papers. A colony of Diabrotica balteata LeConte was allowed to feed on the treated papers, and visitations and feeding responses were recorded. Feeding response was reflected by the darkness of brown stains produced by insect saliva. Visitation frequency was a good indicator of the age of the colony; the parameter was also useful to estimate amounts of cucurbitacin, provided the colony was young. Feeding response declined slightly with increasing age. Dose-response curves showed that concentrations of cucurbitacins could be estimated fairly accurately within the bounds described (4–0.0078 g·l−1). The threshold level was 5 × 10−12 g, when applied in a volume of 0.2 l. Preliminary results indicated that the bioassay can be used to test for the presence of phagodeterrents.
Document Type: Research Article
Publication date: August 1, 1985
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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