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Rearing of the Heliothis Sterile Hybrid with a Multicellular Larval Rearing Container and Pupal Harvesting

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Modification of methods and of a multicellular larval rearing unit for production of Heliothis spp. pupae, particularly the backcross (BC) [BCn ♀ × H. virescens (F.) ♂] from the intercross [H. virescens ♂ × H. subflexa (Guenée) ♀], are described. Essential components of the unit are a fiber glass tray, a polystyrene insert with 903 cells, and a polypropylene cover with 125-m openings. More than 10 million BC pupae have been produced during the last 4 years in Stoneville with this unit.

Optimum egg density was determined to be two to four eggs per cell. Neither benomyl nor folpet affected growth or development of BC larvae when incorporated into the larval diet at rates from 0.25 to 2.0 g/3.8 liter to control contaminants such as the mold Aspergillus niger (Van Tieghew).

The pupal harvesting method collects pupae in a cushioned tray, separates pupae from large particles with a grating, and removes smaller, lighter particles with a highvolume blower.

Document Type: Research Article

Publication date: February 1, 1982

More about this publication?
  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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