Cholinesterase Autoanalysis: a Rapid Method for Biochemical Studies on Susceptible and Resistant Insects

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Abstract:

An automated enzyme assay, which combines ease and speed of operation with high accuracy and reproducibility, was used to investigate activities and inhibition properties of acetylcholinesterases from susceptible and resistant strains of Spodoptera littoralis Boisduval and Musca domestica L. The resistant races of both species were found to contain modified target enzymes characterized by increases in Km and Vmax, and by a decrease in sensitivity against organophosphorus and, to a lesser extent, carbamate insecticides. The different biochemical properties of acetylcholine sterases from susceptible and resistant individuals provide an opportunity for monitoring insect populations before, under, or after selection pressure for frequencies of genes associated with an insensitive target enzyme.

Document Type: Research Article

Publication date: April 1, 1980

More about this publication?
  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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