A Bioassay for Plant-Derived Pest Control Agents Using the European Corn Borer

Authors: Freedman, B.; Nowak, L. J.; Kwolek, W. F.; Berry, E. C.; Guthrie, W. D.

Source: Journal of Economic Entomology, Volume 72, Number 4, August 1979 , pp. 541-545(5)

Publisher: Entomological Society of America

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Abstract:

A bioassay procedure for detecting and evaluating pest control agents in plant extracts has been developed using Ostrinia nubilalis (Hübner). Ground seeds are extracted with petroleum ether and ethanol successively; the extracts are mixed with an artificial diet and tested with 7-day-old larvae. Mortality is generally determined after 3, 9, and 29 days. Data are evaluated by means of computer-facilitated statistical analysis. Those extracts exhibiting significantly higher mortality than controls are ranked on the basis of a rating scale. Of 72 plant species representing 36 families, 12 ethanol extracts and 9 petroleum ether extracts have shown strong toxicity and are being fractionated and tested further. The 3 most toxic ethanol extracts were obtained from Berrya ammonilla Roxb., Thevetia thevetioides (HBK.) K. Schum., and Bassia hyssopifolia (Pall.) Kuntze. The 2 most toxic petroleum ether extracts were obtained from Bauhinia vahlii Wight and Am. and Apium sellowianum H. Wolff. All 5 of these extracts gave 100% larval mortality for the corn borer.

Document Type: Research Article

Publication date: August 1, 1979

More about this publication?
  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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