Growth and Development of the Cheese Skipper Under Sterile, Unsterile, and Sterile-Inoculated Conditions

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Experiments were designed to compare growth and development of Piophila casei (L.) under sterile, unsterile, and sterile-inoculated conditions. A milIipore-filter modification of an egg-sterilization technique was used. In unsterile treatments, eggs were washed only in tap water. For the sterile-inoculated condition, eggs were sterilized and inoculated with 0.5 ml of a 5-h culture of Proteus mutgaris (VPI strain no. 3).

Chicken embryos served as growth medium. Use of the millipore-filter technique elimina.ted the transfer of eggs from one chemical to another, and allowed continual checks of egg survival.

The results indicate that the normal flora accompanying eggs has no beneficial influence on the growth a.nddevelopment of P. casei, and that limiting the number of bacterIa present may be advantageous to the insect. P. vulgaris has very little influence on growth and development of the cheese skipper.

Document Type: Research Article

Publication date: December 1, 1973

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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