Improved Method for Rearing Apple Maggot Larvae on Artificial Media

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Abstract:

Control of contaminants is essential in the artificial rearing of insects on chemical media, and various microbial inhibitors are added to the media for this purpose. These inhibitors must be selected with great care as they may have adverse effects on the insect (Singh and House 1970). This is especially true with the apple maggot, Rhagoletis pomonella (Walsh), as several of the recommended antimicrobial agents interfere with egg development (Neilson 1969) unless the eggs are within one day of hatching when placed on the rearing media (Prokopy 1967). Formalin in the media was not detrimental to eggs or larvae, but it was still unsatisfactory, as the adult progeny seldom mated and laid few or no eggs (Neilson 1969). Formalin in media for rearing the cabbage looper, Trichoplusia ni (Hübner), had no effect on adult reproduction (Vail et al. 1968) and did not affect larval development or survival unless the concentration was at or above 1.0 mg/ml of diet (Ignoffo 1966). Reported here are the effects on larval development and adult reproduction of the apple maggot when the rearing media was autoclaved to suppress contaminants.

Document Type: Research Article

Publication date: April 16, 1973

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