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Determination of Two Juvenile Hormone-Active Compounds and Their Stability in Stable Fly Medium

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In an analytical method for determining 2 juvenile hormone-active Compounds, 4-[6, 7-epoxy-3-ethyl-7-methyl-2-nonenyl)oxy]-1, 2-(methylenedioxy) benzene (ENT-70119) and 6,7-epoxy-l-(p-ethylphenoxy)-3, 7-dimethyl-2-octene (ENT-70221), in a rearing medium for Stomoxys calcitrans(L.), extraction with benzene-methanol (9:1) was followed by column chromatographic cleanup. Quantification of ENT-70221 was by gas chromatography, using flame ionization detection, whereas ENT-70119 was determined by spectrophotofluorometry. Recoveries of compounds added to the medium at 20–1000 ppm ranged from 86 to 99%. The approximate limit of detection was 6 ppm for ENT-702119 and 1 ppm for ENT-70221. On a dry basis, ENT-70119 sprayed as a 0.25% solution on the surface of the medium declined from 1470 ppm after 1 day to 172 ppm 21 days after applieation. Similarly, ENT-70221 sprayed as a 0.5% solution declined from 1620 ppm after 1 day to 288 ppm 22 days after application.

Document Type: Research Article

Publication date: April 16, 1973

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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