Aldrin epoxidation was used as an index of mixed function oxidase activity in gut and fat body homogenates of a caddisfly Lanva, Limnephilus sp., and the transformation of aid rill to dieldrin also was demonstrated in ,vivo. whole larval homogenates were unsuitable enzyme sources, because the gut content contained a soluble. heat-labile inhibitor of epoxidation. Gut and fat body preparations required reduced nicotinamide adenine dinudeotide phosphate for maximum catalytic activity, and epoxidation was reduced by carbon monoxide, sesamex, and SKF 525-A (2- (diethylamine) ethyl 2.2- diphenylvalerate hydrochloride). Bovine serum albumin increased the rate of epoxiclation in gut preparations to a greater extent than it did with fat body preparations. Each homogenate was more active in tris-HCl than in phosphate buffer, and the epoxidase systems were inactivated irreversibly when incubated above 30'.
Document Type: Research Article
Publication date: February 1, 1973
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Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.