Monoamine Oxidase Activity in Larvae of the European Corn Borer1,2,3
Authors: HAYES, D. K.; WASH, D. B.; SCHECHTER, M. S.
Source: Journal of Economic Entomology, Volume 65, Number 5, October 1972 , pp. 1229-1232(4)
Publisher: Entomological Society of America
Abstract:Monoamine oxidase (MAO) activity was determined in homogenates of larvae and pupae of the European corn borer, Ostrinia nubitalis (Hiibner), and the tobacco bud worm, Heliothis virescens (F.), by using 214C-tryptamine as a substrate. Optimum activity was produced by addition of dithiotbreitol to the homogenizing medium and by removing the oxygen from this solution with nitrogen before homogenization. More than 80% of the activity was found in the supernatant fraction of the homogenate resulting from centrifugation at 105,000-g equivalents. Activity was inhibited by addition of s-triazines and by some known MAO inhibitors. Homogenates prepared at different times of day had different levels or activity.
Document Type: Research article
Publication date: 1972-10-01
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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