Temperature-Constant and Temperature Gradient-Free Insectary: Design and Operation
Authors: Klassen, Waldemar; Gentz, Glenn
Source: Journal of Economic Entomology, Volume 64, Number 5, 15 October 1971 , pp. 1334-1336(3)
Publisher: Entomological Society of America
Abstract:The Metabolism and Radiation Research Laboratory is involved in a research program that concerns the environmental control of diapause in insects. We therefore needed a facility in which we could arrange tiers of experimental chambers that would not be exposed to significant variations in temperature in time or space, because temperatures must be controlled to ±1°C if we are to obtain reproducible biological data. For example, in some expensive commercially available environmental chambers, both vertical and horizontal temperature gradients exist. If such chambers were used for studies of photoperiodism, the replicates would have to be tested at the same coordinates of space. In other words, I replicate could not be near the floor of the chamber and 1 near the ceiling. Also, the rearing of insects on artificial diets requires close control of temperature. For example, when larvae of the boll weevil, Anthonomus grandisBoheman, are reared in petri dishes, the diet loses moisture which condenses on the lid if the temperature drops; then these water droplets fall onto the diet and allow mold to grow.
Document Type: Research Article
Publication date: 1971-10-15
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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