Standardization of the δ-Endotoxin Produced by Several Varieties of Bacillus thuringiensis. 2. Enzyme Inhibition (ln50) as a Method of Standardizing Commercial Preparations of the δ-Endotoxin1,2

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The ability of solubilized Bacillus thuringiensis Berliner δ-endotoxin to inhibit bovine pancreatic trypsin was used to determine the toxicity of preparations. When the In50 causing enzymatic inhibition in larval silkworm, Bombyx mori (L.), was plotted on graph paper as the ordinate and the lethal dose (LD50) at 72 hours after oral injection of the δ-endotoxin was plotted as the abscissa, the relationship was found to produce a straight line that can be used to predict the LD50 for silkworm where the In50 of a preparation has been determined.

Document Type: Research Article

Publication date: June 1, 1971

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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