A New Technique For Studying the Toxicology of Insecticides with House Flies by the Infusion Method, with Comparable Topical Application and Injection Results1

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An infusion method is described for administering insecticides into house flies or other insects at constant rates. The calibration of infusion equipment and reproducibility of this method are presented in comparison with topical application and injection results. Because of leakage under infusion pressure. the observed volume of infusion should be calibrated with insects on the needle for each solvent, each infusion time, each rate of infusion, and each insert species. In spite of slow and tedious operation, this technique, together with topical application and injection data, could reveal additional information on the penetration. storage, and detoxication of insecticides in insects. Comparison of the relative activity ratio between topical application and injection and between infusion and injection suggests that, similar to DDT, the site of action of dieldrin on house flies, Musca domestica L.. may be the peripheral nervous system rather than the central nervous system.

Document Type: Research Article

Publication date: February 1, 1971

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