Carbamate Bioassay Using Daphnia magna1

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Abstract:

A bioassay for detection and determination of minute quantities of carbamate insecticides in fruit and vegetable crops was developed with Daphnia magna Straus. The Daphnia bioassay was quick, simple to prepare, reproducible, and proved more sensitive to carbamates than that for Drosophila melanogaster Meigen. Crops were treated in the laboratory and then extracted by a modification of an established method. The extracts of unknowns were evaporated, reconstituted with dimethvl sulfoxide, and injected in 0.025-ml quamities into 13X100 mm test tubes containing 2.5 ml of water. D. magna specimens were introduced, and toxicity observations were taken after 30 minutes. A standard curve was prepared daily in reagent grade solvents; check crop extractions were seldom necessary. Recoveries between 85-100% were consistently obtained.

Document Type: Research Article

Publication date: June 1, 1970

More about this publication?
  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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