Microscopic Examination of Parathion Activating Microsomes from American Cockroach1 Fat Body2,3

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Abstract:

Periplaneta americana (L.) fat body homogenates were separated into 3 fractions by differential centrifugation. The debris fraction (390 gmax for 10 min) supernatant was fractioned to 2 other fractions designated as the mitochondrial (650 gmax for 120 min) and the microsomal (55,610 gmax for 45 min) fractions. The mitochrondrial pellet contained 92% of the cytochrome oxidase and 25% of the parathion activation activity in the debris supernatant. The microsomal fraction contained 17% of the cytochrome oxidase and 77% of the parathion activation activity of the debris supernatant. The microsomal fraction appeared to be derived from the cellular endoplasmic reticulum.

Document Type: Research Article

Publication date: February 1, 1970

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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