Effect of Diluents and Temperature on Instrumental Insemination of Queen Honey Bees1,2,3

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Queen honey bees, Apis mellifera L., were inseminated with 1.25 liter of semen diluted with an equal amount of diluent: 0.9% physiological saline (NaCI) solution containing 0.1% sugar (glucose, fructose, or trehalose or all 3 sugars) or the same sugars or sugar in buffered saline solution (pH 6.9). Unaltered semen, stirred semen, and semen diluted with plain saline solution served as controls. The number of sperm reaching the spermatheca did not differ significantly among treatments (P>0.05). Also when queens were held at 25, 30, or 35 or in the hive for 2 days after insemination with 2 liter of semen, the number of sperm reaching the spermatheca did not differ but when the queens were held at 37.5 and 40 the numbers were significantly lower. The death rate of the queens was high at 40.

Document Type: Research Article

Publication date: December 1, 1969

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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