Effect of Diluents and Temperature on Instrumental Insemination of Queen Honey Bees1,2,3

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Queen honey bees, Apis mellifera L., were inseminated with 1.25 liter of semen diluted with an equal amount of diluent: 0.9% physiological saline (NaCI) solution containing 0.1% sugar (glucose, fructose, or trehalose or all 3 sugars) or the same sugars or sugar in buffered saline solution (pH 6.9). Unaltered semen, stirred semen, and semen diluted with plain saline solution served as controls. The number of sperm reaching the spermatheca did not differ significantly among treatments (P>0.05). Also when queens were held at 25, 30, or 35 or in the hive for 2 days after insemination with 2 liter of semen, the number of sperm reaching the spermatheca did not differ but when the queens were held at 37.5 and 40 the numbers were significantly lower. The death rate of the queens was high at 40.

Document Type: Research Article

Publication date: December 1, 1969

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